Intersouth Partners continues to mine the Washington, D.C., area for new investments, leading a $4.5 million first round for Defywire, a provider of wireless enterprise software solutions.

Anthem Capital Management and The Washington Dinner Club joined Intersouth in the round for Defywire, which is based in Reston, Va. Intersouth venture partner Don Rainey and Xander Perry of Anthem Capital Management will join Defywire’s board of directors.

“I’ve seen countless emerging companies over the years and few have accomplished what Defywire has been able to do in just over 12 months,” Rainey said in a statement. “The software meets an urgent market need, the industry has validated the product, and the management team is experienced at returning significant value to investors. Simply put, all the pieces are there.”

Over the past year, Defywire has signed major partnership and distribution deals with webMethods, Sun Microsystems and Sharp Electronics. Its software has been implemented in Fortune 1000 companies, including GE Access, and the company launched its server-based wireless software solution.

The company’s Java-based architecture links mobile executives with business-critical enterprise applications – securely and in real time – that historically have only been accessed via a landline connection. The software allows users to access leading enterprise resource planning packages, databases, messaging software, sales force automation systems and customer relationship management software.

Defywire marks Intersouth’s third investment in the Washington and Baltimore area this year. It also led a $4.75 million first round for Baltimore-based Artifact Software and participated in a $17 million round for Covega, an optical components maker in Jessup, Md.

Intersouth Partners: